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Investigational Research on an Optimum Procedure for Trophectoderm Biopsy

Introduction

For many years, cleavage stage biopsy has been the common approach to obtain human embryonic cells for PGD (Harper et al., 2012). After opening the zona pellucida by the use of a 1.48 μm microsur¬gical laser or by mechanical zona dissection, one or two blastomeres are aspirated and processed for subsequent analysis of numerical or structural chromosome aber¬rations or single gene disorders. De Vos et al. (2009) clearly showed that the removal of two blastomeres from a day 3 embryo (corresponding to a minimum loss of 25 % of the embryonic mass) compromises treatment outcome in terms of live birth rate. The study could not show corresponding effects for single blastomere biopsy, however, its impact on further embryonic development and differentiation is still being discussed.

An alternative approach to day 3 biopsy is….

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