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Techniques to achieve low oxygen tension for culture of human embryos

The debate over whether human embryos should be maintained in vitro under physiological oxygen concentrations, similar to that estimated for in vivo oviduct and uterine tissue (e.g. approximately 5 to 10%), or whether they should be cultured using an atmospheric oxygen tension of around 20%, is ongoing and largely unresolved. The premise of this presentation is that the embryologist desires to culture human embryos at physiologic oxygen concentrations, and would like to explore the mechanical options available to achieve this endpoint.

Discussion on the effects of oxygen tension on embryonic development in vitro must include…

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