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Challenging Traditional Embryo Culture Techniques with a Simplified, Continuous Single Medium Protocol

Mammalian preimplantation embryos develop in vitro with some degree of plasticity, as evidenced by the varied culture media and techniques in use today. There are a number of commercial media formulations for stage-specific use; media for fertilization, sequential media for growth of fertilized ova, and complex single medium formulations designed to present all components to the embryos during all stages of post-fertilization in vitro development. The historical progression of culture media, discussions of culture media components, and a brief history of media…

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