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Improved Embryo Development and Comparable Pregnancy Rates Using Single vs. Sequential Culture Media from the Day of Oocyte Retrieval to the Blastocysts Stage of Development

Introduction

Throughout the 30 plus year history of human IVF there has been a remarkable increase in the length of time embryos are held in the incubator (from two to six days) before returning them to the uterus, without compromising their ability to initiate a pregnancy (1). Part of the reason for this enhanced embryo viability outside the female reproductive system has been the improvements in culture technology, including culture media (2-4).

A significant milestone in human embryo culture was achieved in the late 1990s with the introduction…

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