Our Advertisers

Use of Oil Overlay in the Culture of Embryos

Covering small volumes of culture media (50 to 100ml) with oil is a commonly used technique for culturing human gametes and embryos. It was first used for culture of embryos by Gwatkin (1963) and then further described by Brinster (1963); however, other culture methods using oil overlay were explored during the early days of embryo culture (Brinster, 1968). Oil overlay was used in the culture of the first successful IVF offspring in the human being (Edwards et al., 1969, Edwards et al., 1980) and monkey (Bavister et al., 1983). This practice allows small volumes, microdrops, of…

[issuu width=930 height=700 pageNumber=9 shareMenuEnabled=false printButtonEnabled=false backgroundColor=%23222222 documentId=120125173944-d3d5a2d865184b8a84a5ec4f7e317958 name=jce-14-1 username=embryologists.com tag=clinical unit=px id=92692f17-260b-e48a-e34f-13626b50b8f4 v=2]