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PCR-based Assays for Human Immunodeficiency, Hepatitis B, C and Cytomegalo Viruses and Treponema pallidum in Semen

Commercially available PCR-based assays for infectious agents in semen are lacking. To understand semen burden of HIV, we developed a triple bracket- nested RT-PCR assay that is highly specific and sensitive to 20 copies of HIV RNA, and one infected cell. We have adapted this assay to detect Hepatitis C RNA, Hepatitis B DNA and CMV DNA, to sensitivities of approximately 200, 200 and 10 copies, respectively, of World Health Organization (WHO) standards. We previously reported a PCR-based survey of abundant…

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