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Rapid Soluble HLA-G ELISA for Analyzing Day 2 Spent Embryo Culture Media

Introduction
The soluble Human Leukocyte Antigen-G (sHLA-G) produced by the human embryo may be one of the most promising markers for the prediction of success following In Vitro Fertilization and Embryo Transfer (IVFET) procedure(1). Despite the fact that many studies have identified sHLA-G in the spent culture media and reported its correlation to higher cleavage rate and pregnancy success following IVFET (1-7), controversy still exists (8, 9). The controversy may be due to the differences in assay techniques employed, embryo culture media type, culture conditions, and volume used…

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