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The Effect of Embryo Culture System on In-Vitro Development and Outcome of Transfers

Introduction

An effective embryo culture system is essential to the success of an assisted reproduction program. Early attempts to develop defined embryo culture media focused on attempting to mimic the components of oviduct fluid at their measured concentrations. For human embryos, the most notable example was human tubal fluid (HTF), as described by Quinn and his colleagues (Quinn et al. 1984; Quinn et al. 1985). Although this approach seems intuitively logical, the physical conditions of in-vitro culture are very different from those in the oviduct. Consequently, it…

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